The population of Uzbekistan, around in 1994 at about 23 million, is the largest of the Central Asian republics, comprising more than 40 % of their total population. Growing at a rapid rate, the population is split by ethnic and regional differences. The Russian component of the population shrank steadily in the years after freedom.

Uzbeks make up about three-fourths of the population, followed by Russians, Tajiks, Tatars, Kyrgyz, Ukrainians, Kazaks, and Karakalpaks. The Uzbeks speak a language belonging to the southeastern, or Chagatai (Turki), branch of the Turkic language group. The Uzbeks are Sunnite Muslims, and they are considered to be among the most devout Muslims in all of Central Asia. They are also the least Russified of the Turkic peoples formerly under Soviet rule, and virtually all of them still claim Uzbek as their primary language. The majority of Uzbeks live in rural areas. Two-fifths of the population of Uzbekistan lives in urban areas; the urban population has a disproportionately high number of non-Uzbeks. Slavic peoples—Russians, Ukrainians, and Belarusians—held a large proportion of administrative positions. In the late 1980s and early '90s, many Russians and smaller numbers of Jews emigrated from Uzbekistan and other Central Asian states, changing the ethnic balance and employment patterns in the region.